2000-2003
BS in Botany, Chemistry and Zoology, University of Allahabad, India
2004-2006
MS in Botany, Banaras Hindu University, Varanasi, India
2007-2012
Jawaharlal Nehru University, New Delhi, India
2013-2018
Postdoctoral Fellow, Washington University School of Medicine, Saint Louis, USA
2019-
Senior Scientist,Washington University School of Medicine, Saint Louis, USA
Research
Biochemical and biophysical characterization of Pif1-family of helicases, mitochondrial single-stranded DNA binding proteins and DNA polymerases.
Publications
Singh SP, Soranno A, Sparks MA, Galletto R.
Branched unwinding mechanism of the Pif1 family of DNA helicases.
Proc Natl Acad Sci USA 2019 Dec 3. doi: 10.1073/pnas.1915654116. PubMed PMID: 31744872.
Sparks MA, Singh SP, Burgers PM, Galletto R.
Complementary roles of Pif1 helicase and single stranded DNA binding proteins in stimulating DNA replication through G-quadruplexes.
Nucleic Acids Res. 2019 Jul 24. pii: gkz608. doi:10.1093/nar/gkz608.
Singh SP, Kukshal V, Galletto R.
A stable tetramer is not the only oligomeric state that mitochondrial single-stranded DNA binding proteins can adopt.
J Biol Chem. 2019 Mar 15;294(11):4137-4144. doi: 10.1074/jbc.RA118.007048.
Dahan D, Tsirkas I, Dovrat D, Sparks MA, Singh SP, Galletto R, Aharoni A.
Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures.
Nucleic Acids Res. 2018 Dec 14;46(22):11847-11857. doi:10.1093/nar/gky1065.
Geronimo CL, Singh SP, Galletto R, Zakian VA.
The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity.
Nucleic Acids Res. 2018 Sep 19;46(16):8357-8370. doi: 10.1093/nar/gky655.
Singh SP, Kukshal V, De Bona P, Antony E, Galletto R.
The mitochondrial single-stranded DNA binding protein from S. cerevisiae, Rim1, does not formstable homo-tetramers and binds DNA as a dimer of dimers.
Nucleic Acids Res. 2018 Aug 21;46(14):7193-7205. doi: 10.1093/nar/gky530.
Koc KN, Singh SP, Stodola JL, Burgers PM, Galletto R.
Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ.
Nucleic Acids Res. 2016 May 5;44(8):3811-9. doi: 10.1093/nar/gkw181.
Singh SP, Koc KN, Stodola JL, Galletto R.
A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3′-End.
J Mol Biol. 2016 Mar 27;428(6):1053-1067. doi:10.1016/j.jmb.2016.02.017.